mutant es cell resource Search Results


mutant es cell resource  (International Mouse Phenotyping Consortium)
90
International Mouse Phenotyping Consortium mutant es cell resource
Mutant Es Cell Resource, supplied by International Mouse Phenotyping Consortium, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mutant es cell resource/product/International Mouse Phenotyping Consortium
Average 90 stars, based on 1 article reviews
mutant es cell resource - by Bioz Stars, 2026-03
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ak7  (Mutant Mouse Resource & Research Center)
90
Mutant Mouse Resource & Research Center ak7
Ak7, supplied by Mutant Mouse Resource & Research Center, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ak7/product/Mutant Mouse Resource & Research Center
Average 90 stars, based on 1 article reviews
ak7 - by Bioz Stars, 2026-03
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sanger mirko es cell line mir342 ( mir342 tm1wtsi  (Mutant Mouse Resource & Research Center)
90
Mutant Mouse Resource & Research Center sanger mirko es cell line mir342 ( mir342 tm1wtsi
The metabolic phenotypes of <t>Mir342</t> (-/-) and Mir342 (+/+) mice fed with high fat-high sucrose (HFHS) or standard (STD) chow. (A) The HFHS/STD ratio of miRNA read numbers in epididymal fat tissues. (B) Body and tissue weight of Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS (n=7) or STD chow (n=8). (C) Adipocyte area in epididymal and subdermal adipose tissues (n=4). Quantitative analyses were carried out on PAS-stained paraffin sections. (D) Glucose tolerance test (GTT) and insulin tolerance test (ITT) of mice fed with HFHS (n=7) or STD chow (n=8). (E) Serum insulin levels in Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS chow during GTT (n=4). (F) Fasting serum leptin levels in Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS mice (n=4). Data shown as mean ± SD and analyzed by independent t -test or two-way ANOVA with Bonferroni tests (*p < 0.05; **p < 0.01).
Sanger Mirko Es Cell Line Mir342 ( Mir342 Tm1wtsi, supplied by Mutant Mouse Resource & Research Center, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sanger mirko es cell line mir342 ( mir342 tm1wtsi/product/Mutant Mouse Resource & Research Center
Average 90 stars, based on 1 article reviews
sanger mirko es cell line mir342 ( mir342 tm1wtsi - by Bioz Stars, 2026-03
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s. aureus mutant strains of cell wall-anchored proteins (cwas)  (BEI Resources)
90
BEI Resources s. aureus mutant strains of cell wall-anchored proteins (cwas)
The metabolic phenotypes of <t>Mir342</t> (-/-) and Mir342 (+/+) mice fed with high fat-high sucrose (HFHS) or standard (STD) chow. (A) The HFHS/STD ratio of miRNA read numbers in epididymal fat tissues. (B) Body and tissue weight of Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS (n=7) or STD chow (n=8). (C) Adipocyte area in epididymal and subdermal adipose tissues (n=4). Quantitative analyses were carried out on PAS-stained paraffin sections. (D) Glucose tolerance test (GTT) and insulin tolerance test (ITT) of mice fed with HFHS (n=7) or STD chow (n=8). (E) Serum insulin levels in Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS chow during GTT (n=4). (F) Fasting serum leptin levels in Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS mice (n=4). Data shown as mean ± SD and analyzed by independent t -test or two-way ANOVA with Bonferroni tests (*p < 0.05; **p < 0.01).
S. Aureus Mutant Strains Of Cell Wall Anchored Proteins (Cwas), supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/s. aureus mutant strains of cell wall-anchored proteins (cwas)/product/BEI Resources
Average 90 stars, based on 1 article reviews
s. aureus mutant strains of cell wall-anchored proteins (cwas) - by Bioz Stars, 2026-03
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Image Search Results


The metabolic phenotypes of Mir342 (-/-) and Mir342 (+/+) mice fed with high fat-high sucrose (HFHS) or standard (STD) chow. (A) The HFHS/STD ratio of miRNA read numbers in epididymal fat tissues. (B) Body and tissue weight of Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS (n=7) or STD chow (n=8). (C) Adipocyte area in epididymal and subdermal adipose tissues (n=4). Quantitative analyses were carried out on PAS-stained paraffin sections. (D) Glucose tolerance test (GTT) and insulin tolerance test (ITT) of mice fed with HFHS (n=7) or STD chow (n=8). (E) Serum insulin levels in Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS chow during GTT (n=4). (F) Fasting serum leptin levels in Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS mice (n=4). Data shown as mean ± SD and analyzed by independent t -test or two-way ANOVA with Bonferroni tests (*p < 0.05; **p < 0.01).

Journal: Frontiers in Endocrinology

Article Title: Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control

doi: 10.3389/fendo.2021.727915

Figure Lengend Snippet: The metabolic phenotypes of Mir342 (-/-) and Mir342 (+/+) mice fed with high fat-high sucrose (HFHS) or standard (STD) chow. (A) The HFHS/STD ratio of miRNA read numbers in epididymal fat tissues. (B) Body and tissue weight of Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS (n=7) or STD chow (n=8). (C) Adipocyte area in epididymal and subdermal adipose tissues (n=4). Quantitative analyses were carried out on PAS-stained paraffin sections. (D) Glucose tolerance test (GTT) and insulin tolerance test (ITT) of mice fed with HFHS (n=7) or STD chow (n=8). (E) Serum insulin levels in Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS chow during GTT (n=4). (F) Fasting serum leptin levels in Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS mice (n=4). Data shown as mean ± SD and analyzed by independent t -test or two-way ANOVA with Bonferroni tests (*p < 0.05; **p < 0.01).

Article Snippet: To further give a new insight and investigate the role of Mir342 in obesity and diabetes, we obtained Sanger MirKO ES cell line Mir342 ( Mir342 tm1Wtsi ) from MMRRC (Mutant Mouse Resource & Research Centers) and generated Mir342 knockout mice [ Mir342 (-/-)].

Techniques: Staining

Correlation of serum mmu-mir-342-3p levels ( Log Mir342 ) with various clinical parameters in the patients with type 2 diabetes (n=65). (A) In correlation matrix, Spearman’s rank correlation coefficient is shown. *P < 0.05. (B) The correlations between Log miR-342-3p (Log Mir342 ) with HbA1c (R=0.222, p=0.076), body weight (R=0.280, p=0.024) and body mass index (R=0.248, p=0.052).

Journal: Frontiers in Endocrinology

Article Title: Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control

doi: 10.3389/fendo.2021.727915

Figure Lengend Snippet: Correlation of serum mmu-mir-342-3p levels ( Log Mir342 ) with various clinical parameters in the patients with type 2 diabetes (n=65). (A) In correlation matrix, Spearman’s rank correlation coefficient is shown. *P < 0.05. (B) The correlations between Log miR-342-3p (Log Mir342 ) with HbA1c (R=0.222, p=0.076), body weight (R=0.280, p=0.024) and body mass index (R=0.248, p=0.052).

Article Snippet: To further give a new insight and investigate the role of Mir342 in obesity and diabetes, we obtained Sanger MirKO ES cell line Mir342 ( Mir342 tm1Wtsi ) from MMRRC (Mutant Mouse Resource & Research Centers) and generated Mir342 knockout mice [ Mir342 (-/-)].

Techniques:

Food intake of Mir342 (-/-) and Mir342 (+/+) mice fed with high fat-high sucrose (HFHS) or standard (STD) chow. (A) Daily food intake over 3 consecutive days of mice fed with HFHS (n=9) or STD chow (n=8) at 18 weeks of age. (B) Average food intake over 3 days per g body weight of mice fed with HFHS (n=9) or STD chow (n=8). (C) Pair-feeding studies in Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS chow (n=5). Free-feeding mice (n=3 in each group) were used as controls to match the food intake. Data shown as mean ± SD and analyzed by independent t -test or two-way ANOVA with Bonferroni tests (*p < 0.05; **p < 0.01).

Journal: Frontiers in Endocrinology

Article Title: Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control

doi: 10.3389/fendo.2021.727915

Figure Lengend Snippet: Food intake of Mir342 (-/-) and Mir342 (+/+) mice fed with high fat-high sucrose (HFHS) or standard (STD) chow. (A) Daily food intake over 3 consecutive days of mice fed with HFHS (n=9) or STD chow (n=8) at 18 weeks of age. (B) Average food intake over 3 days per g body weight of mice fed with HFHS (n=9) or STD chow (n=8). (C) Pair-feeding studies in Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS chow (n=5). Free-feeding mice (n=3 in each group) were used as controls to match the food intake. Data shown as mean ± SD and analyzed by independent t -test or two-way ANOVA with Bonferroni tests (*p < 0.05; **p < 0.01).

Article Snippet: To further give a new insight and investigate the role of Mir342 in obesity and diabetes, we obtained Sanger MirKO ES cell line Mir342 ( Mir342 tm1Wtsi ) from MMRRC (Mutant Mouse Resource & Research Centers) and generated Mir342 knockout mice [ Mir342 (-/-)].

Techniques:

Expression of Mir342 and its host gene Evl . (A) Mir342 is an intronic miRNA in Evl (Enabled/Vasodilator-stimulated phosphoprotein) gene. (B, C) In various tissues, the expression of miR-342-3p is normalized by snoRNA202 and snoRNA234, while Evl is normalized by Rplp0 and Rn18s . HFHS Mir342 (+/+) (n=4), HFHS Mir342 (-/-) (n=3), STD Mir342 (+/+) (n=4) and STD Mir342 (-/-) mice (n=4). Bar=100 μm. Data are analyzed by one-way ANOVA with a Tukey test. (D) Western blot analyses and quantification of EVL protein levels of brain tissue normalized by β-actin (ACTB). Data are analyzed by independent t -test. (E–H) In in situ hybridization, the sections of hypothalamus from Mir342 (+/+) mice were hybridized with Mir342 probe ( E ; Mir342 ), U6 snRNA probe ( F ; U6 ), and Scramble-miR probe ( G ; Scramble ). The inset in (E) is shown in panel (H) . Immunostaining of EVL (red) in hypothalamus of Mir342 (+/+) mice (I) and nuclear staining of DAPI (blue) (J) are shown. Bars are 100 and 50 μm in panels (E, H) , respectively. Data presented as means ± SD (*p < 0.05; **p < 0.01).

Journal: Frontiers in Endocrinology

Article Title: Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control

doi: 10.3389/fendo.2021.727915

Figure Lengend Snippet: Expression of Mir342 and its host gene Evl . (A) Mir342 is an intronic miRNA in Evl (Enabled/Vasodilator-stimulated phosphoprotein) gene. (B, C) In various tissues, the expression of miR-342-3p is normalized by snoRNA202 and snoRNA234, while Evl is normalized by Rplp0 and Rn18s . HFHS Mir342 (+/+) (n=4), HFHS Mir342 (-/-) (n=3), STD Mir342 (+/+) (n=4) and STD Mir342 (-/-) mice (n=4). Bar=100 μm. Data are analyzed by one-way ANOVA with a Tukey test. (D) Western blot analyses and quantification of EVL protein levels of brain tissue normalized by β-actin (ACTB). Data are analyzed by independent t -test. (E–H) In in situ hybridization, the sections of hypothalamus from Mir342 (+/+) mice were hybridized with Mir342 probe ( E ; Mir342 ), U6 snRNA probe ( F ; U6 ), and Scramble-miR probe ( G ; Scramble ). The inset in (E) is shown in panel (H) . Immunostaining of EVL (red) in hypothalamus of Mir342 (+/+) mice (I) and nuclear staining of DAPI (blue) (J) are shown. Bars are 100 and 50 μm in panels (E, H) , respectively. Data presented as means ± SD (*p < 0.05; **p < 0.01).

Article Snippet: To further give a new insight and investigate the role of Mir342 in obesity and diabetes, we obtained Sanger MirKO ES cell line Mir342 ( Mir342 tm1Wtsi ) from MMRRC (Mutant Mouse Resource & Research Centers) and generated Mir342 knockout mice [ Mir342 (-/-)].

Techniques: Expressing, Western Blot, In Situ Hybridization, Immunostaining, Staining

The activation of neuropeptide Y (NPY) and proopiomelanocortin (POMC) neurons by leptin injection. (A) Representative photographs of NPY (Green) and EVL (Red) double staining in arcuate nuclei from Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS chow (n=5 each). The arrows indicate double-positive cells. The percentage and total numbers of NPY + EVL + cells are shown. (B) NPY (Green) and pSTAT3 (Red) double staining in the mice fed with HFHS (n=4) after intraperitoneal injection of leptin (1 mg/kg body weight). The percentage and total numbers of NPY + pSTAT3 + cells are shown. (C) Double staining with POMC (Green) and EVL (Red) in Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS chow (n=5). The percentage and total numbers of POMC + EVL + cells are shown. (D) POMC (Green) and pSTAT3 (Red) double staining in the mice fed with HFHS (n=4) after intraperitoneal injection of leptin (1 mg/kg body weight). The percentage and total numbers of POMC + pSTAT3 + cells are shown. (E) Average cell numbers of NPY + (n=9) and POMC + (n=9) cells detected in hypothalamus of the mice fed with HFHS. (F) Average cell numbers of EVL + (n=10) and pSTAT3 + (n=8) cells detected in hypothalamus of the mice fed with HFHS. Data shown as mean ± SD and analyzed by independent t -test (*p < 0.05; **p < 0.01).

Journal: Frontiers in Endocrinology

Article Title: Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control

doi: 10.3389/fendo.2021.727915

Figure Lengend Snippet: The activation of neuropeptide Y (NPY) and proopiomelanocortin (POMC) neurons by leptin injection. (A) Representative photographs of NPY (Green) and EVL (Red) double staining in arcuate nuclei from Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS chow (n=5 each). The arrows indicate double-positive cells. The percentage and total numbers of NPY + EVL + cells are shown. (B) NPY (Green) and pSTAT3 (Red) double staining in the mice fed with HFHS (n=4) after intraperitoneal injection of leptin (1 mg/kg body weight). The percentage and total numbers of NPY + pSTAT3 + cells are shown. (C) Double staining with POMC (Green) and EVL (Red) in Mir342 (+/+) and Mir342 (-/-) mice fed with HFHS chow (n=5). The percentage and total numbers of POMC + EVL + cells are shown. (D) POMC (Green) and pSTAT3 (Red) double staining in the mice fed with HFHS (n=4) after intraperitoneal injection of leptin (1 mg/kg body weight). The percentage and total numbers of POMC + pSTAT3 + cells are shown. (E) Average cell numbers of NPY + (n=9) and POMC + (n=9) cells detected in hypothalamus of the mice fed with HFHS. (F) Average cell numbers of EVL + (n=10) and pSTAT3 + (n=8) cells detected in hypothalamus of the mice fed with HFHS. Data shown as mean ± SD and analyzed by independent t -test (*p < 0.05; **p < 0.01).

Article Snippet: To further give a new insight and investigate the role of Mir342 in obesity and diabetes, we obtained Sanger MirKO ES cell line Mir342 ( Mir342 tm1Wtsi ) from MMRRC (Mutant Mouse Resource & Research Centers) and generated Mir342 knockout mice [ Mir342 (-/-)].

Techniques: Activation Assay, Injection, Double Staining

The expression and reporter assay of Snap25 (synaptosomal-associated protein, 25kDa). (A) Relative mRNA expression of Snap25 normalized by Rplp0 and Rn18s in brain and epididymal fat tissues detected by RT-qPCR. (B) Western blot analyses and quantification of SNAP25 protein levels in hypothalamus. (C) Dual-luciferase reporter assay. pmirGLO-Snap25 WT 3’-UTR, pmirGLO-Snap25 MT 3’-UTR, and pmirGLO no-insert control plasmids were cotransfected with Mir342 mimic, Mir342 inhibitor, negative control siRNA (mimic NC), inhibitor negative control (inhibitor NC) into HEK293T cells, respectively. (D) The expression of predicted target genes (Fat2, Msi1 and Nhlh2) in brain. Data are analyzed by independent t-test or one-way ANOVA with a Tukey test. All data are presented as mean ± SD (*p < 0.05; **p < 0.01).

Journal: Frontiers in Endocrinology

Article Title: Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control

doi: 10.3389/fendo.2021.727915

Figure Lengend Snippet: The expression and reporter assay of Snap25 (synaptosomal-associated protein, 25kDa). (A) Relative mRNA expression of Snap25 normalized by Rplp0 and Rn18s in brain and epididymal fat tissues detected by RT-qPCR. (B) Western blot analyses and quantification of SNAP25 protein levels in hypothalamus. (C) Dual-luciferase reporter assay. pmirGLO-Snap25 WT 3’-UTR, pmirGLO-Snap25 MT 3’-UTR, and pmirGLO no-insert control plasmids were cotransfected with Mir342 mimic, Mir342 inhibitor, negative control siRNA (mimic NC), inhibitor negative control (inhibitor NC) into HEK293T cells, respectively. (D) The expression of predicted target genes (Fat2, Msi1 and Nhlh2) in brain. Data are analyzed by independent t-test or one-way ANOVA with a Tukey test. All data are presented as mean ± SD (*p < 0.05; **p < 0.01).

Article Snippet: To further give a new insight and investigate the role of Mir342 in obesity and diabetes, we obtained Sanger MirKO ES cell line Mir342 ( Mir342 tm1Wtsi ) from MMRRC (Mutant Mouse Resource & Research Centers) and generated Mir342 knockout mice [ Mir342 (-/-)].

Techniques: Expressing, Reporter Assay, Quantitative RT-PCR, Western Blot, Luciferase, Negative Control